The other two women are being treated for shock. She did not release more details about the victims, including where in the United States the tourists live. She spoke on condition of anonymity, per the French judicial system.
The prosecutor's office could not be reached for comment but told France 3 that one of the women had been hit in the eye with acid and had trouble seeing. The spokeswoman told the AP that the suspect did not make extremist threats.
French police say it is too early to determine if the incident was terrorism related. Police said the attack was not terror-related. The city is just over three hours away from Paris by train. The two women reportedly sprayed with acid remain in hospital.
US authorities in France are not immediately commenting on what happened to protect the privacy of the American tourists, embassy spokesman Alex Daniels said.
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The tourists were hospitalized after the attack.
Marseille is located on France's southern coast, about 490 miles from Paris.
French police arrested a 41-year-old woman in connection to the attack, according to the Associated Press, which cited an anonymous source in the local prosecutor's office.